Ultraviolet 320 nm laser excitation for flow cytometry
نویسندگان
چکیده
منابع مشابه
Simultaneous analysis of the cyan, yellow and green fluorescent proteins by flow cytometry using single-laser excitation at 458 nm.
BACKGROUND Development of spectrally distinct green fluorescent protein (GFP) variants has allowed for simultaneous flow cytometric detection of two different colored mutants expressed in a single cell. However, the dual-laser methods employed in such experiments are not widely applicable since they require a specific, expensive laser, and single-laser analysis at 488 nm exhibits considerable s...
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Lasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air-cooled argon-ion laser emitting 10-25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers. Also included is a discussion of future directions, particularly in the development of solid-...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2017
ISSN: 1552-4922
DOI: 10.1002/cyto.a.23066